Article

Characterisation of the transcriptome and proteome of SARS-CoV-2 using direct RNA sequencing and tandem mass spectrometry reveals evidence for a cell passage induced in-frame deletion in the spike glycoprotein that removes the furin-like cleavage site

2020-03-24

Abstract excerpt

<h4>ABSTRACT</h4> Direct RNA sequencing using an Oxford Nanopore MinION characterised the transcriptome of SARS-CoV-2 grown in Vero E6 cells. This cell line is being widely used to propagate the novel coronavirus. The viral transcriptome was analysed using a recently developed ORF-centric pipeline. This revealed the pattern of viral transcripts, (i.e. subgenomic mRNAs), generally fitted the predicted replication...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 7f91f24e-6174-51f8-acc2-a6631550ea19
DOI: 10.1101/2020.03.22.002204

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Characterisation of the transcriptome and proteome of SARS-CoV-2 using direct RNA sequencing and tandem mass spectrometry reveals evidence for a cell passage induced in-frame deletion in the spike glycoprotein that removes the furin-like cleavage siteDOI 10.1101/2020.03.22.002204Read

Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoproteinSemantic proximity Read

Furin Cleavage Site Is Key to SARS-CoV-2 PathogenesisSemantic proximity Read

QTQTN motif upstream of the furin-cleavage site plays a key role in SARS-CoV-2 infection and pathogenesisSemantic proximity Read

Sequential Glycosylations at the Multibasic Cleavage Site of SARS-CoV-2 Spike Protein Regulate Viral Activation, Assembly, and InfectionSemantic proximity Read

Naturally occurring SARS-CoV-2 gene deletions close to the spike S1/S2 cleavage site in the viral quasispecies of COVID19 patientsSemantic proximity Read

Spike glycoprotein and host cell determinants of SARS-CoV-2 entry and cytopathic effectsSemantic proximity Read

QTQTN motif upstream of the furin-cleavage site plays key role in SARS-CoV-2 infection and pathogenesisSemantic proximity Read

The SARS-CoV-2 transcriptome and the dynamics of the S gene furin cleavage site in primary human airway epitheliaSemantic proximity Read

Meta-transcriptomic analysis reveals the gene expression and novel conserved sub-genomic RNAs in SARS-CoV-2 and MERS-CoVSemantic proximity Read

Pre-pandemic artificial MERS analog of pathogenic polyfunctional SARS-CoV-2 S1/S2 furin cleavage site domain is unique among betacoronavirusesSemantic proximity Read

Identification of Common Deletions in the Spike Protein of Severe Acute Respiratory Syndrome Coronavirus 2Semantic proximity Read

The architecture of SARS-CoV-2 transcriptomeSemantic proximity Read