Article

Translation initiation by the Kozak mRNA sequence is based on a conformational readout on the ribosome

2025-07-12

Abstract excerpt

The recognition mechanism of Kozak mRNA, typically comprising purines in the - 3 and +4 positions flanking the AUG start codon, has remained enigmatic for decades. To address this fundamental function in eukaryotes during translation initiation, we analysed the cryo-EM structures of human 48S preinitiation complexes with mRNA point mutations. They reveal a fan-like intercalation of the pre-codon triplet into the 1...

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Literature Corpus work: 7bc2395d-d4ec-5247-ba78-1dd8c0786514
DOI: 10.1101/2025.07.11.664391

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