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Article

Highly Parallel Profiling of Cas9 Variant Specificity.

Molecular cell - 21 May 2020

Schmid-Burgk Jonathan L, Gao Linyi, Li David, Gardner Zachary, Strecker Jonathan, Lash Blake, Zhang Feng

Abstract excerpt

Determining the off-target cleavage profile of programmable nucleases is an important consideration for any genome editing experiment, and a number of Cas9 variants have been reported that improve specificity. We describe here tagmentation-based tag integration site sequencing (TTISS), an efficient, scalable method for analyzing double-strand breaks (DSBs) that we apply in parallel to eight Cas9 variants across...

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Identifiers

PMID: 32187529
DOI: 10.1016/j.molcel.2020.02.023
PMCID: PMC7370240

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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