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High-throughput robotic isolation of human iPS cell clones reveals frequent homozygous induction of identical genetic manipulations by CRISPR-Cas9

2024-09-19

Abstract excerpt

<h4>SUMMARY</h4> Genome editing in human iPS cells is a powerful approach in regenerative medicine. CRISPR-Cas9 is the most common genome editing tool, but it often induces byproduct insertions and deletions in addition to the desired edits. Therefore, genome editing of iPS cells produces diverse genotypes. Existing assays mostly analyze genome editing results in cell populations, but not in single cells. However...

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Literature Corpus work: 73acf507-87c7-5399-9c09-0f02c6d28779
DOI: 10.1101/2024.09.18.613641

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