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Integration of Xeno-Free Single-cell Cloning in CRISPR-mediated DNA Editing of Human iPSCs Improves Homogeneity and Methodological Efficiency of Cellular Disease Modelling

2022-04-13

Abstract excerpt

<h4>SUMMARY</h4> The capability to generate induced pluripotent stem cell (iPSC) lines, in tandem with CRISPR-Cas9 DNA editing, offers great promise to understand the underlying genetic mechanisms of human disease. The low efficiency of available methods for homogeneous expansion of singularised CRISPR-transfected iPSCs necessitates the coculture of transfected cells in mixed populations and/or on feeder layers....

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Literature Corpus work: 56f4fb7b-6a6d-5e9e-acdc-b288680fb1b8
DOI: 10.1101/2022.04.12.488094

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