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Single-stranded DNA library preparation uncovers the origin and diversity of ultrashort cell-free DNA in plasma

2015-12-30

Abstract excerpt

Circulating cell-free DNA (cfDNA) is emerging as a powerful monitoring tool in cancer, pregnancy and organ transplantation. Nucleosomal DNA, the predominant form of cfDNA in blood, can be readily adapted for sequencing via ligation of double-stranded DNA (dsDNA) adapters. dsDNA library preparation, however, is insensitive to ultrashort, degraded and single-stranded cfDNA. Drawing inspiration from recent technical...

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Literature Corpus work: 72f3487f-0649-5319-ac7d-1b1f027dc4a3
DOI: 10.1101/035741

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