Article

Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data

2017-07-18

Abstract excerpt

<h4>Background</h4> Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some steps in the procedure, such as PCR amplification, are known to introduce biases, a systematic evaluation of...

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Literature Corpus work: 71059172-714d-5ae5-9e3e-ad30801e5538
DOI: 10.1101/165449

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