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CRISPR-Cas9-mediated depletion of <i>O</i> -GlcNAc hydrolase and transferase for functional dissection of <i>O</i> -GlcNAcylation in human cells

2020-08-20

Abstract excerpt

O -GlcNAcylation is an abundant post-translational modification (PTM) on serine and threonine residues of nuclear and cytoplasmic proteins. Although this PTM has been reported on thousands of proteins, O -GlcNAc transferase (OGT) and hydrolase (OGA) are the only two enzymes that perform the respective addition and removal of O -GlcNAc on protein substrates. To examine the consequences of deregulated O -GlcNAcyl...

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Literature Corpus work
692814ca-682f-5c28-9438-1d02a2526998
DOI
10.1101/2020.08.19.258079
Open publication

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