Article

Reengineering the Specificity of the Highly Selective <i>Clostridium botulinum</i> Protease via Directed Evolution

2020-09-30

Abstract excerpt

The botulinum neurotoxin serotype A (BoNT/A) cuts a single peptide bond in SNAP25, an activity used to treat a wide range of diseases. Reengineering the substrate specificity of BoNT/A’s protease domain (LC/A) could expand its therapeutic applications; however, LC/A’s extended substrate recognition (≈60 residues) challenges conventional approaches. We report a directed evolution method for retargeting LC/A’s subst...

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Identifiers and source

Literature Corpus work: 641866ce-ab65-5aee-814f-77ac4c81ef2d
DOI: 10.1101/2020.09.29.319145

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