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Article

Phage-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity.

Science (New York, N.Y.) - 19 Feb 2021

Blum Travis R, Liu Hao, Packer Michael S, Xiong Xiaozhe, Lee Pyung-Gang, Zhang Sicai, Richter Michelle, Minasov George, Satchell Karla J F, Dong Min, Liu David R

Abstract excerpt

Although bespoke, sequence-specific proteases have the potential to advance biotechnology and medicine, generation of proteases with tailor-made cleavage specificities remains a major challenge. We developed a phage-assisted protease evolution system with simultaneous positive and negative selection and applied it to three botulinum neurotoxin (BoNT) light-chain proteases. We evolved BoNT/X protease into separate...

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Identifiers

PMID: 33602850
DOI: 10.1126/science.abf5972
PMCID: PMC8175023

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