Article
AI-redesigned starting points and outcomes enhance protein evolution.
Nature - 1 Sept 2026
Krasnow Nicholas A, Xu Joy A, Zhang Emily, Mahadeshwar Gandhar K, Tao Y Allen, McCreary Julia, Hemez Colin F, Brown Logan E, Jiang Wei, Liu David R
Abstract excerpt
Engineered or laboratory-evolved proteins often have suboptimal stability, activity or specificity. Here we applied artificial intelligence (AI)-based protein sequence design to address challenges in experimental enzyme evolution. Using the model ProteinMPNN, we redesigned three distinct botulinum neurotoxin (BoNT) proteases, generating variants with improved stability and full catalytic efficiency1. We...
Read the complete abstract on PubMedTopics
- Directed Molecular Evolution
- Models, Molecular
- Protein Engineering
- Artificial Intelligence
- Mutation
- Evolution, Molecular