Article

High-resolution NMR spectroscopy of proteins in intact mitochondria

2025-03-19

Abstract excerpt

Understanding protein structure and function within mitochondria is essential for unraveling the molecular mechanisms underlying cellular energy production, stress response, and disease. Here, we present a novel approach for NMR observation of proteins within intact mitochondria by delivering proteins directly into isolated mitochondria via electroporation. Using this method, we investigate the interaction of α-sy...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 5faf3fdd-6945-5238-8b19-2dc09b1b7477
DOI: 10.1101/2025.03.19.644076

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

High-resolution NMR spectroscopy of proteins in intact mitochondriaDOI 10.1101/2025.03.19.644076Read

A new split-GFP-based probe reveals DJ-1 translocation into the mitochondrial matrix to sustain ATP synthesis upon nutrient deprivation.Semantic proximity Read

Unfolding is the driving force for mitochondrial import and degradation of the Parkinson's disease-related protein DJ-1Semantic proximity Read

Rationally Designed Variants of α-Synuclein Illuminate Its in vivo Structural Properties in Health and DiseaseSemantic proximity Read

Structural conversion of α-synuclein at the mitochondria induces neuronal toxicitySemantic proximity Read

Alpha-synuclein fibrils induce budding of mitochondrial-derived vesiclesSemantic proximity Read

The lipid-binding domain of wild type and mutant alpha-synuclein: compactness and interconversion between the broken and extended helix forms.Semantic proximity Read

Mitochondrial protein interaction landscape of SS-31Semantic proximity Read

Structural basis of membrane disruption and cellular toxicity by α-synuclein oligomers.Semantic proximity Read

Concentration-dependent mutational scanning probes the cellular folding landscape of α-synuclein in yeast.Semantic proximity Read

Concentration-Dependent Mutational Scanning Probes the Cellular Folding Landscape of <i>α</i> -Synuclein in YeastSemantic proximity Read

Robust Sequence Determinants of α-Synuclein Toxicity in Yeast Implicate Membrane BindingSemantic proximity Read

Regulation of Exocytosis and Mitochondrial Relocalization by Alpha-Synuclein in a Mammalian Cell ModelSemantic proximity Read