Article

Evidence for negative selection against somatic mutations in fibroblasts with high N-ethyl-N-nitrosourea-induced mutation load

2024-04-07

Abstract excerpt

Advanced sequencing has revealed that thousands of mutations accumulate with age in most human tissues. While some can clonally expand and cause disease, the maximum mutation load a cell can tolerate without functional decline is unknown. We addressed this by repeatedly treating proliferating human primary fibroblasts with a low dose of the mutagen N-ethyl-N-nitrosourea and quantifying somatic mutation burden usin...

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Literature Corpus work: 511c3feb-70f7-5d9e-b781-91aa5e1b2faa
DOI: 10.1101/2024.04.07.588286

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