Article

A rapid agarose-free protocol for preparing human organotypic lung cultures to study respiratory virus infection and evaluate antivirals <i>ex vivo</i>

2026-02-13

Abstract excerpt

<h4>Summary</h4> We describe a standardized and reproducible procedure to generate human organotypic lung cultures from surgical lung resection for the study of respiratory infections. The protocol details tissue harvesting, biopsy punching, mechanical slicing, culture at the air–liquid interface. This technique enables robust ex vivo infections of human lung tissue with respiratory viruses, including Influenza...

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Literature Corpus work: 508a4804-7943-599d-9b5c-79c9c9307ff1
DOI: 10.64898/2026.02.12.705542

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