Article

Protocol for primary human lung organoid-derived air-liquid interface <i>in vitro</i> model to study response to SARS-CoV-2

2023-09-11

Abstract excerpt

<h4>Summary</h4> This article presents a comprehensive protocol for establishing primary human lung organoid-derived air-liquid interface (ALI) cultures from cryopreserved human lung tissue. These cultures serve as a physiologically relevant model to study human airway epithelium in vitro . The protocol encompasses lung tissue cryostorage, tissue dissociation, lung epithelial organoid generation, and ALI culture...

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Literature Corpus work: 91f35cdd-f3ed-58c5-b86a-3ca3870f363b
DOI: 10.1101/2023.09.10.557067

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