Anti-CRISPR protein mediated degradation of Cas9 in human cells

Abstract excerpt

Bacteriophages encode anti-CRISPR (Acr) proteins that inactivate CRISPR-Cas bacterial immune systems, allowing successful invasion, replication, and prophage integration. Acr proteins inhibit CRISPR-Cas systems using a wide variety of mechanisms. AcrIIA1 is encoded by numerous phages and plasmids, binds specifically to the Cas9 HNH domain, and was the first Acr discovered to inhibit SpyCas9. Here we report the obs...

Topics

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR and Genetic Engineering
• CRISPR-Associated Proteins
• CRISPR-Cas Systems
• Directed Molecular Evolution
• Nucleotide Motifs
• Protein Engineering
• Staphylococcus aureus

Identifiers and source

Literature Corpus work

4ffd2f3f-b3e0-51ae-830c-cd9900167944

DOI

10.1101/2023.03.29.534776