Article

Mechanism of broad-spectrum Cas9 inhibition by AcrIIA11

2021-09-15

Abstract excerpt

Mobile genetic elements evade CRISPR-Cas adaptive immunity by encoding anti-CRISPR proteins (Acrs). Acrs inactivate CRISPR-Cas systems via diverse mechanisms but are generally specific for a narrow subset of Cas nucleases that share high sequence similarity. Here, we demonstrate that AcrIIA11 inhibits diverse Cas9 sub-types in vitro and human cells. Single-molecule fluorescence imaging reveals that AcrIIA11 inter...

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Literature Corpus work: d123f13b-2526-598d-8362-8a26419db50d
DOI: 10.1101/2021.09.15.460536

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