Article

Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome

2019-03-06

Abstract excerpt

CRISPR-Cas systems protect bacteria and archaea from phages and other mobile genetic elements, which use small anti-CRISPR (Acr) proteins to overcome CRISPR-Cas immunity. Because they are difficult to identify, the natural diversity and impact of Acrs on microbial ecosystems is underappreciated. To overcome this discovery bottleneck, we developed a high-throughput functional selection that isolates acr genes base...

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Literature Corpus work: 2d44e8dd-c1fb-5fee-a2ea-dba80824fe8e
DOI: 10.1101/569095

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