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Multidose Transient Transfection of HEK293 Cells Modulates rAAV2/5 Rep Protein Expression and Influences the Enrichment Fraction of Filled Capsids

2024-03-16

Abstract excerpt

Recombinant adeno-associated virus (rAAV) is a commonly used in vivo gene therapy vector because of its non-pathogenicity, long-term transgene expression, broad tropism, and ability to transduce both dividing and non-dividing cells. However, rAAV vector production via transient transfection of mammalian cells typically yields a low fraction of filled-to-total capsids (~1–30% of total capsids produced). Analysis o...

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Literature Corpus work: 4eb36a12-f84b-5726-a368-7ce1c79e7b09
DOI: 10.22541/au.171062191.10777478/v1

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Multidose Transient Transfection of HEK293 Cells Modulates rAAV2/5 Rep Protein Expression and Influences the Enrichment Fraction of Filled CapsidsDOI 10.22541/au.171062191.10777478/v1Read

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