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Perfusion‐Based Production of rAAV via an Intensified Transient Transfection Process

18 Mar 2025

Abstract excerpt

Increasing demand for recombinant adeno-associated virus (rAAV)-based gene therapies necessitates increased manufacturing production. Transient transfection of mammalian cells remains the most commonly used method to produce clinical-grade rAAVs due to its ease of implementation. However, transient transfection processes are often characterized by suboptimal yields and low fractions of full-to-total capsids, both...

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PMID: 40103325
DOI: 10.1002/bit.28967

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