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Producing high-quantity and high-quality recombinant adeno-associated virus by low-cis triple transfection

12 Mar 2024

Abstract excerpt

Recombinant adeno-associated virus (rAAV)-based gene therapy is entering clinical and commercial stages at an unprecedented pace. Triple transfection of HEK293 cells is currently the most widely used platform for rAAV manufacturing. Here, we develop low-cis triple transfection that decreases transgene plasmid use by 10- to 100-fold and overcomes several major limitations associated with standard triple...

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PMID: 38558570
DOI: 10.1016/j.omtm.2024.101230

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