A method to estimate the frequency of chromosomal rearrangements induced by CRISPR/Cas9 multiplexing in Drosophila

Abstract excerpt

Using CRISPR/Cas9 to simultaneously induce mutations in two or more target genes, commonly referred to as multiplexing, may result in chromosomal rearrangements such as inversions or translocations. While this may be undesirable in some contexts, the ability to recover chromosomal rearrangements targeted to specific sites in the genome is potentially a powerful tool. Before developing such tools, however, it is fi...

Topics

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• Germ Cells
• Animals, Genetically Modified
• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Cas Systems
• Drosophila
• Embryo, Nonmammalian
• Gene Knock-In Techniques
• RNA, Guide, CRISPR-Cas Systems

Identifiers and source

Literature Corpus work

4a90464e-93be-5c26-830e-6c5c615cb57f

DOI

10.1101/815431