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Article

CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells.

Nature protocols - 1 Oct 2020

Xia Baolong, Amador Gabriel, Viswanatha Raghuvir, Zirin Jonathan, Mohr Stephanie E, Perrimon Norbert

Abstract excerpt

Precise and efficient genome modifications provide powerful tools for biological studies. Previous CRISPR gene knockout methods in cell lines have relied on frameshifts caused by stochastic insertion/deletion in all alleles. However, this method is inefficient for genes with high copy number due to polyploidy or gene amplification because frameshifts in all alleles can be difficult to generate and detect. Here we...

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Identifiers

PMID: 32958931
DOI: 10.1038/s41596-020-0383-8
PMCID: PMC7961850

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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