Back to search

Article

ACE2-independent SARS-CoV-2 infection and mouse adaption emerge after passage in cells expressing human and mouse ACE2

2021-12-21

Abstract excerpt

<h4>SUMMARY</h4> Human ACE2 (hACE2) is the key cell attachment and entry receptor for SARS-CoV-2, with the original SARS-CoV-2 isolates unable to use mouse ACE2 (mACE2). Herein we describe a new system for generating mouse-adapted SARS-CoV-2 in vitro by serial passaging virus in co-cultures of cell lines expressing hACE2 and mACE2. Mouse-adapted viruses emerged with up to five amino acid changes in the spike pro...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
46b3fd6a-f808-589f-8665-2077eef5bfc0
DOI
10.1101/2021.12.16.473063
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
ACE2-independent SARS-CoV-2 infection and mouse adaption emerge after passage in cells expressing human and mouse ACE2DOI 10.1101/2021.12.16.473063
Evolution of ACE2-independent SARS-CoV-2 infection and mouse adaption after passage in cells expressing human and mouse ACE2Semantic proximity
ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsSemantic proximity
A mouse-adapted SARS-CoV-2 strain replicating in standard laboratory miceSemantic proximity
A mouse-adapted SARS-CoV-2 model for the evaluation of COVID-19 medical countermeasuresSemantic proximity
Characterization and structural basis of a lethal mouse-adapted SARS-CoV-2Semantic proximity
Comparative analysis reveals the species-specific genetic determinants of ACE2 required for SARS-CoV-2 entrySemantic proximity
Human ACE2 Gene Replacement Mice Support SARS-CoV-2 Viral Replication and Nonlethal Disease ProgressionSemantic proximity
Determinants of susceptibility to SARS-CoV-2 infection in murine ACE2.Semantic proximity
Characterization and structural basis of a lethal mouse-adapted SARS-CoV-2Semantic proximity
Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cellSemantic proximity
Comparative analysis reveals the species-specific genetic determinants of ACE2 required for SARS-CoV-2 entrySemantic proximity
Cell type-specific adaptation of the SARS-CoV-2 spikeSemantic proximity
Select a neighboring publication to make it the new centre.