Article

Bridge recombinase enables versatile rewriting of bacterial genomes

2026-04-29

Abstract excerpt

Bacteria drive crucial processes across ecosystems and impact human health, yet tools to rewrite microbiomes remain limited. Here, we show that bridge recombinase enables versatile and programmable genome editing across the bacterial tree of life. In Escherichia coli , we achieved 142 kb insertions at >90% efficiency, megabase-scale inversions (2.3 Mb), and pathway-scale 50 kb excisions. With a single ortholog an...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 22c2551c-9a1d-5c30-a7b4-f603b4098956
DOI: 10.64898/2026.04.29.721476

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Bridge recombinase enables versatile rewriting of bacterial genomesDOI 10.64898/2026.04.29.721476Read

Megabase-scale human genome rearrangement with programmable bridge recombinasesSemantic proximity Read

Large-scale discovery of recombinases for integrating DNA into the human genomeSemantic proximity Read

Transformation of Natural Genetic Variation into Haemophilus Influenzae GenomesSemantic proximity Read

Development of a recombineering system for the acetogen <i>Eubacterium limosum</i> with Cas9 counterselection for markerless genome engineeringSemantic proximity Read

Parallel tracking by sequencing reveals the impact of restriction modification systems on transfer of an integrative and conjugative elementSemantic proximity Read

High-throughput functional variant screens via in vivo production of single-stranded DNA.Semantic proximity Read

In vivo generation of DNA sequence diversity for cellular barcoding.Semantic proximity Read

RecT recombinase expression enables efficient gene editing in <i>Enterococcus</i>Semantic proximity Read

Targeted Genome Editing of Bacteria Within Microbial CommunitiesSemantic proximity Read

Continuous Multiplexed Phage Genome Editing Using RecombitronsSemantic proximity Read

A self-propagating, barcoded transposon system for the dynamic rewiring of genomic networks.Semantic proximity Read

Improved bacterial recombineering by parallelized protein discoverySemantic proximity Read