Article
Detecting Full-Length EccDNA with FLED and long-reads sequencing
2023-06-24
Abstract excerpt
Reconstructing the full-length sequence of extrachromosomal circular DNA (eccDNA) from short sequencing reads has proved challenging given the similarity of eccDNAs and their corresponding linear DNAs. Previous sequencing methods were unable to achieve high-throughput detection of full-length eccDNAs. Here we describe a new strategy that combined rolling circle amplification (RCA) and nanopore long-reads sequencin...
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Identifiers and source
- Literature Corpus work
- 03e0dc05-e32b-59ce-9b9d-5989a9336ffa
- DOI
- 10.1101/2023.06.21.545840