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A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

26 Feb 2022

Abstract excerpt

BACKGROUND: Technological advancements in the era of massive parallel sequencing have enabled the functional dissection of the human transcriptome. However, 5' ends of mRNAs are significantly underrepresented in these datasets, hindering the efficient analysis of the complex human transcriptome. The implementation of the template-switching mechanism at the reverse transcription stage along with 5' rapid...

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PMID: 35219290
DOI: 10.1186/s12864-022-08386-y

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