Article

Engineered mitochondrial A-to-G editors with enhanced efficiency and targeting scope

2024-11-20

Abstract excerpt

<h4>Summary</h4> Mitochondrial base editing is a powerful technology, but the current A-to-G mitochondrial DNA (mtDNA) base editors are reluctant to achieve high efficiency, which is critical for mtDNA engineering. Through laboratory evolution, TadA-8e variants were discovered with substantially increased activity and expanded targeting compatibility, especially for previously unfavorite sequence contexts, when a...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 3de3b4b9-13ef-58b4-b326-f7cd23af70a2
DOI: 10.1101/2024.11.19.623682

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Engineered mitochondrial A-to-G editors with enhanced efficiency and targeting scopeDOI 10.1101/2024.11.19.623682Read

A mitochondrial disease model is generated and corrected using engineered base editors in rat zygotes.Semantic proximity Read

Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNASemantic proximity Read

One-step generation of heritable mitochondrial DNA multiplex-engineered rats using DddA-derived cytosine base editor.Semantic proximity Read

Structure-guided engineering of AI-derived adenine base editors for nuclear and mitochondrial DNA editing.Semantic proximity Read

Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian cells and embryos.Semantic proximity Read

Engineering a precise adenine base editor with minimal bystander editing.Semantic proximity Read

Precise modelling of mitochondrial diseases using optimized mitoBEs.Semantic proximity Read

Unconstrained Precision Mitochondrial Genome Editing with αDdCBEsSemantic proximity Read

Engineering precise adenine base editor with infinitesimal rates of bystander mutations and off-target editingSemantic proximity Read

Enhanced C-To-T and A-To-G Base Editing in Mitochondrial DNA with Engineered DdCBE and TALED.Semantic proximity Read

Engineering targeted deletions in the mitochondrial genomeSemantic proximity Read

The FusX TALE Base Editor (FusXTBE) for rapid mitochondrial DNA programming of human cells <i>in vitro</i> and zebrafish disease models <i>in vivo</i>Semantic proximity Read