Article

An approach for accelerated isolation of genetically manipulated cell clones with reduced clonal variability

2018-12-31

Abstract excerpt

Genomic editing methods, such as the CRISPR/Cas9 system, are routinely used to study gene function in somatic cells. Due to the heterogeneity of mutations, it is necessary to purify cell clones grown in high dilution until colony formation, which can be a time consuming process. Here we tested a modified approach in which we seeded cells in high dilution, together with non-edited carrier cells. As a comparison, ce...

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Literature Corpus work: 3c782448-b730-56a5-9627-fd046c0df263
DOI: 10.1101/508721

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