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Utilizing Stable Gene‐Edited Knockout Pools for Genetic Screening and Engineering in Chinese Hamster Ovary Cells

1 May 2025

Abstract excerpt

Chinese hamster ovary (CHO) cells are the primary host for biopharmaceutical production. To meet increasing demands for productivity, quality, and complex molecule expression, genetic engineering, particularly clustered regularly interspaced short palindromic repeats (CRISPR)-mediated gene knockout (KO), is widely used to optimize host cell performance. However, systematic screening of KO targets remains...

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PMID: 40376717
DOI: 10.1002/biot.70033

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