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Article

Clonally Selected Lines After CRISPR-Cas Editing Are Not Isogenic.

The CRISPR journal - 1 Apr 2023

Panda Arijit, Suvakov Milovan, Mariani Jessica, Drucker Kristen L, Park Yohan, Jang Yeongjun, Kollmeyer Thomas M, Sarkar Gobinda, Bae Taejeong, Kim Jean J, Yoon Wan Hee, Jenkins Robert B, Vaccarino Flora M, Abyzov Alexej

Abstract excerpt

The CRISPR-Cas9 system has enabled researchers to precisely modify/edit the sequence of a genome. A typical editing experiment consists of two steps: (1) editing cultured cells; (2) cell cloning and selection of clones with and without intended edit, presumed to be isogenic. The application of CRISPR-Cas9 system may result in off-target edits, whereas cloning will reveal culture-acquired mutations. We analyzed...

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Identifiers

PMID: 37071670
DOI: 10.1089/crispr.2022.0050
PMCID: PMC10123805

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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