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Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation

30 Jul 2018

Abstract excerpt

Mammalian cells are widely used to express genes for basic biology studies and biopharmaceuticals. Current methods for generation of engineered cell lines introduce high genomic and phenotypic diversity, which hamper studies of gene functions and discovery of novel cellular mechanisms. Here, we minimized clonal variation by integrating a landing pad for recombinase-mediated cassette exchange site-specifically...

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PMID: 30060646
DOI: 10.1021/acssynbio.8b00140

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