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An optimized genome-wide, virus-free CRISPR screen for mammalian cells

1 Aug 2021

Abstract excerpt

Pooled CRISPR screens have been widely applied to mammalian and other organisms to elucidate the interplay between genes and phenotypes of interest. The most popular method for delivering the CRISPR components into mammalian cells is lentivirus based. However, because lentivirus is not always an option, virus-free protocols are starting to emerge. Here, we demonstrate an improved virus-free, genome-wide CRISPR...

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PMID: 34935002
DOI: 10.1016/j.crmeth.2021.100062

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