Article

Buffer Valency Engineering Enables High-concentration and Shelf-stable DNA Transfection Particles for Viral Vector Production

2025-07-05

Abstract excerpt

Cost-effective and scalable production is critical for advancing the clinical translation of adeno-associated virus (AAV)-mediated gene therapy. The widely used transient transfection method using plasmid DNA (pDNA)-loaded transfection particles for AAV production faces technical challenges due to instability of the particles and the concentration limits for particle preparation, hindering reproducibility and scal...

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Literature Corpus work: 38582e77-ce62-59e9-8534-550744bb524c
DOI: 10.1101/2025.07.02.662322

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