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Buffer Valency Engineering Enables High-concentration and Shelf-stable DNA Transfection Particles for Viral Vector Production

2025-10-23

Abstract excerpt

<title>Abstract</title> <p>Cost-effective and scalable production is critical for advancing the clinical translation of viral vector-mediated gene therapy. The widely used transient transfection method for the production of adeno-associated virus (AAVs) and lentivirus utilizes polyethyleneimine (PEI)/DNA particles loaded with multiple plasmids; however, the current pDNA/PEI particles must be prepared under dilute...

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Literature Corpus work: b9ba1f46-198f-56eb-ac54-98e1daae1fdb
DOI: 10.21203/rs.3.rs-7042059/v1

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