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Towards a scalable bioprocess for rAAV production using a HeLa stable cell line

7 Apr 2023

Abstract excerpt

Abstract The majority of recombinant adeno‐associated viruses (rAAV) approved for clinical use or in clinical trials areproduced by transient transfection using the HEK293 cell line. However, this platform has several manufacturing bottlenecks at commercial scales namely, low product quality (full to empty capsid ratio <20% in most rAAV serotypes), lower productivities obtained after scale‐up and the high cost of...

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PMID: 37027346
DOI: 10.1002/bit.28394

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