The tandem affinity purification (TAP) method: a general procedure of protein complex purification.

doi:10.1006/meth.2001.1183

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Article

The tandem affinity purification (TAP) method: a general procedure of protein complex purification.

Methods (San Diego, Calif.) - 1 Jul 2001

Puig O, Caspary F, Rigaut G, Rutz B, Bouveret E, Bragado-Nilsson E, Wilm M, Séraphin B

Abstract excerpt

Identification of components present in biological complexes requires their purification to near homogeneity. Methods of purification vary from protein to protein, making it impossible to design a general purification strategy valid for all cases. We have developed the tandem affinity purification (TAP) method as a tool that allows rapid purification under native conditions of complexes, even when expressed at...

Topics

Bacterial Proteins
Blotting, Western
DNA, Bacterial
Fungal Proteins
Genetic Vectors
Methods
Mutation
Polymerase Chain Reaction
Proteins
Proteome
Ribonucleases
Ribonucleoproteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Staphylococcus aureus

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Open in PubMed

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PMID: 11403571
DOI: 10.1006/meth.2001.1183

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