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Article

The tandem affinity purification (TAP) method: a general procedure of protein complex purification.

Methods (San Diego, Calif.) - 1 Jul 2001

Puig O, Caspary F, Rigaut G, Rutz B, Bouveret E, Bragado-Nilsson E, Wilm M, Séraphin B

Abstract excerpt

Identification of components present in biological complexes requires their purification to near homogeneity. Methods of purification vary from protein to protein, making it impossible to design a general purification strategy valid for all cases. We have developed the tandem affinity purification (TAP) method as a tool that allows rapid purification under native conditions of complexes, even when expressed at...

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PMID: 11403571
DOI: 10.1006/meth.2001.1183

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