Article

Primer- and template-independent RNA polymerization by terminal nucleotidyltransferase TENT4B

2026-03-05

Abstract excerpt

RNA synthesis by eukaryotic polymerases requires existing polynucleotides to serve as templates or primers. Here, we describe primer- and template-free RNA generation by human terminal nucleotidyltransferase 4B (TENT4B) via de novo polymerization of free nucleotides. We observed that recombinant TENT4B (rTENT4B) consumes ATP to yield inorganic pyrophosphate in the absence of a primer or template, concurrent with t...

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Literature Corpus work: 2b517126-75aa-54e6-ac55-e9989fa7f26d
DOI: 10.64898/2026.03.05.709691

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