Article
Terminal phosphate labeled nucleotides: synthesis, applications, and linker effect on incorporation by DNA polymerases.
Nucleosides, nucleotides & nucleic acids - 1 Jan 2005
Kumar Shiv, Sood Anup, Wegener Jeffery, Finn Patrick J, Nampalli Satyam, Nelson John R, Sekher Anuradha, Mitsis Paul, Macklin John, Fuller Carl W
Abstract excerpt
A number of terminal phosphate-labeled nucleotides with three or more phosphates and with varied length linkers attached between the terminal phosphate and the dye have been synthesized. These nucleotides have been tested as substrates for different DNA and RNA polymerases. We have also explored their utility in DNA sequencing, SNP analysis, nucleic acid amplification, quantitative PCR, and other biochemical assays.
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