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Engineering a Thermostable Reverse Transcriptase for RT-PCR Through Rational Design of Pyrococcus furiosus DNA Polymerase.

Biomolecules - 24 Oct 2025

Kuznetsova Aleksandra A, Grishina Irina A, Mikushina Elena S, Kuznetsov Nikita A

Abstract excerpt

Engineering of a bifunctional enzyme that combines DNA-dependent DNA polymerase and reverse transcriptase (RT) activities is a highly promising biotechnological goal, as it would enable one-enzyme RT-PCR. For this purpose, we selected the high-fidelity Pyrococcus furiosus (Pfu) DNA polymerase as engineering scaffold. The selection of amino acid residues for replacement was carried out based on a multi-sequence...

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Amino Acid Sequence
DNA-Directed DNA Polymerase
Enzyme Stability
Protein Engineering
Pyrococcus furiosus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Reverse Transcriptase Polymerase Chain Reaction

RNA-Directed DNA Polymerase

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PMID: 41301425
DOI: 10.3390/biom15111507
PMCID: PMC12650659

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