Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Single-base discrimination mediated by proofreading 3' phosphorothioate-modified primers.

Molecular biotechnology - 1 Nov 2003

Zhang Jia, Li Kai

Abstract excerpt

It has been well known for decades that deoxyribonucleic acid (DNA) polymerases with proofreading function have a higher fidelity in primer extension as compared to those without 3' exonuclease activities. However, polymerases with proofreading function have not been used in single nucleotide polymorphism (SNP) assays. Here, we describe a new method for single-base discrimination by proofreading the 3'...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA Primers
Share to Deoxyribonucleotides
Share to DNA-Directed DNA Polymerase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Polymerase I

Share to Exonucleases

Share to Templates, Genetic

Share to Thermodynamics

Share to Thionucleotides

Source

Identifiers

PMID: 14668536
DOI: 10.1385/MB:25:3:223

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Single-base discrimination mediated by proofreading 3' phosphorothioate-modified primers.PMID 14668536Read ↗

Enhanced discrimination of single nucleotide polymorphism in genotyping by phosphorothioate proofreading allele-specific amplification.Semantic proximity Read ↗

Different applications of polymerases with and without proofreading activity in single-nucleotide polymorphism analysis.Semantic proximity Read ↗

Single base extension (SBE) with proofreading polymerases and phosphorothioate primers: improved fidelity in single-substrate assays.Semantic proximity Read ↗

SNP discrimination through proofreading and OFF-switch of exo+ polymerase.Semantic proximity Read ↗

[Novel mechanism of 3' exonuclease of polymerase in maintenance of DNA replication fidelity and its application in SNP assay].Semantic proximity Read ↗

Single-base discrimination mediated by proofreading inert allele specific Primers.Semantic proximity Read ↗

Proofreading genotyping assays mediated by high fidelity exo+ DNA polymerases.Semantic proximity Read ↗

Dissecting and Tuning Primer Editing by Proofreading PolymerasesSemantic proximity Read ↗

Increased single-nucleotide discrimination in allele-specific polymerase chain reactions through primer probes bearing nucleobase and 2'-deoxyribose modifications.Semantic proximity Read ↗

Exo+ proofreading polymerases mediate genetic analysis and its application in biomedical studies.Semantic proximity Read ↗

A primerless molecular diagnostic: phosphorothioated-terminal hairpin formation and self-priming extension (PS-THSP).Semantic proximity Read ↗

Touchdown thermocycling program enables a robust single nucleotide polymorphism typing method based on allele-specific real-time polymerase chain reaction.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.