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Article

Method for Rapid Analysis of Mutant RNA Polymerase Activity on Templates Containing Unnatural Nucleotides.

International journal of molecular sciences - 14 May 2021

Egorova Tatiana, Shuvalova Ekaterina, Mukba Sabina, Shuvalov Alexey, Kolosov Peter, Alkalaeva Elena

Abstract excerpt

Pairs of unnatural nucleotides are used to expand the genetic code and create artificial DNA or RNA templates. In general, an approach is used to engineer orthogonal systems capable of reading codons comprising artificial nucleotides; however, DNA and RNA polymerases capable of recognizing unnatural nucleotides are required for amplification and transcription of templates. Under favorable conditions, in the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34069057
DOI: 10.3390/ijms22105186
PMCID: PMC8155940

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