Article
Enzymatic synthesis of random sequences of RNA and RNA analogues by DNA polymerase theta mutants for the generation of aptamer libraries.
Nucleic acids research - 6 Jul 2018
Randrianjatovo-Gbalou Irina, Rosario Sandrine, Sismeiro Odile, Varet Hugo, Legendre Rachel, Coppée Jean-Yves, Huteau Valérie, Pochet Sylvie, Delarue Marc
Abstract excerpt
Nucleic acid aptamers, especially RNA, exhibit valuable advantages compared to protein therapeutics in terms of size, affinity and specificity. However, the synthesis of libraries of large random RNAs is still difficult and expensive. The engineering of polymerases able to directly generate these libraries has the potential to replace the chemical synthesis approach. Here, we start with a DNA polymerase that...
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