Mutational analysis of the human Xbp1 translational arrest peptide and construction of arrest-enhanced variants

Abstract excerpt

Xbp1, a protein involved in the unfolded protein response, is a rare example of a mammalian protein that contains a well-defined translational arrest peptide (AP). In order to define the critical residues in the Xbp1u AP, and to search for variants with stronger arrest potency than the wildtype Xbp1u AP, we have carried out a full mutagenesis scan where each residue in the AP was replaced by the other 19 natural a...

Topics

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• Biomechanical Phenomena
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Mutagenesis, Site-Directed
• Peptide Chain Elongation, Translational
• Peptide Elongation Factors
• Peptides
• Ribosomes

Identifiers and source

Literature Corpus work

2927dc10-c7f1-5f79-b882-9c65348b4c6e

DOI

10.1101/201913