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Article

Production of human β-actin and a mutant using a bacterial expression system with a cold shock vector.

Protein expression and purification - 1 Jul 2011

Tamura Minoru, Ito Katsunori, Kunihiro Sachio, Yamasaki Chihoko, Haragauchi Mihoko

Abstract excerpt

Actin is the most abundant protein in the cytoplasm of most eukaryotic cells and is involved in a variety of cellular functions. It has been difficult to produce actin in bacterial expression systems in good yields. In this study, we developed a new simple method for the production of recombinant actin in Escherichia coli cells. Human β-actin was successfully expressed using a cold shock vector, pCold, in the...

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Identifiers

PMID: 20851184
DOI: 10.1016/j.pep.2010.09.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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