Article

Population scale nucleic acid delivery to <i>Caenorhabditis elegans</i> via electroporation

2020-10-15

Abstract excerpt

<h4>ABSTRACT</h4> The free-living nematode C.elegans remains one of the most robust and flexible genetic systems for inter-rogating the complexities of animal biology. Targeted genetic manipulations, such as RNA interference (RNAi), CRISPR/Cas9- or array-based transgenesis, all depend on initial delivery of nucleic acids. Delivery of dsRNA by feeding can be effective, but expression in E. coli is not conducive...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a5f3f06a-9a86-525c-90d7-4c385bc710b6
DOI: 10.1101/2020.10.15.340513

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Population scale nucleic acid delivery to <i>Caenorhabditis elegans</i> via electroporationDOI 10.1101/2020.10.15.340513Read

A simple method to dramatically increase <i>C. elegans</i> germline microinjection efficiencySemantic proximity Read

High-efficiency CRISPR gene editing in <i>C. elegans</i> using Cas9 integrated into the genomeSemantic proximity Read

Rapid generation of <i>C. elegans</i> single copy transgenes combining RMCE and drug selectionSemantic proximity Read

Gene silencing by double-stranded RNA from <i>C. elegans</i> neurons reveals functional mosaicism of RNA interferenceSemantic proximity Read

A microfluidic device for automated, high-speed microinjection of <i>Caenorhabditis elegans</i>Semantic proximity Read

Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans.Semantic proximity Read

Modular safe-harbor transgene insertion (MosTI) for targeted single-copy and extrachromosomal array integration in <i>C. elegans</i>Semantic proximity Read

Transposon Regulation in the <i>Caenorhabditis elegans</i> Germline and SomaSemantic proximity Read

Liposome-based transfection enhances RNAi and CRISPR-mediated mutagenesis in non-model nematode systemsSemantic proximity Read

Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes.Semantic proximity Read

FLInt: single shot safe harbor transgene integration via Fluorescent Landmark Interference.Semantic proximity Read

A Robust Chemical-free Platform for Age-Synchronized <i>Caenorhabditis elegans</i> Populations Maintenance for High-Throughput Screening in Aging StudiesSemantic proximity Read