Article

Base-editing a single missense mutation in A20 enhances CAR-T cell efficacy

2025-10-06

Abstract excerpt

T cell exhaustion limits the efficacy of cancer immunotherapies. Here, we performed genome-wide loss-of-function screening in repetitively stimulated human T cells and identified the mulitfunctional ubiquitin-modifying protein A20/TNFAIP3 as a major negative regulator of exhausted T cell persistence. Protein large language modeling, deep base-editing mutagenesis, and studies in immunocompetent mice with domain-spe...

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Literature Corpus work: 2681f76b-3832-5f49-985f-704f6bc337f5
DOI: 10.1101/2025.10.05.680562

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