Article

Optimized single molecule fluorescence sheds light on elusive enzymatic mechanisms

2020-01-16

Abstract excerpt

Single molecule enzymology using fluorescent substrate requires truly minimal amounts of proteins. This is highly beneficial when the protein source is either advanced expression systems or samples from humans/animals with ethical and economic implications. Further benefits of single molecule analysis is the potential to reveal phenomena hidden in ensemble studies. However, dye photophysics and fluorescent contami...

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Literature Corpus work: 25d8293a-b849-5c72-8753-2a9672836cdc
DOI: 10.1101/2020.01.16.908947

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