Article

MethylCal: Bayesian calibration of methylation levels

2019-03-24

Abstract excerpt

Bisulfite amplicon sequencing has become the primary choice for single-base methylation quantification of multiple targets in parallel. The main limitation of this technology is a preferential amplification of an allele and strand in the PCR due to methylation state. This effect, known as “PCR bias”, causes inaccurate estimation of the methylation levels and calibration methods based on standard controls have been...

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Literature Corpus work: 257a0410-3a5d-5db9-a55f-5039793dda93
DOI: 10.1101/587519

Open publication

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