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Comprehensive comparison of enzymatic and bisulfite DNA methylation analysis in clinically relevant samples

3 Oct 2025

Abstract excerpt

BACKGROUND: Bisulfite conversion is considered the gold standard for DNA methylation analysis, but it damages DNA and performs sub-optimally with clinical samples (e.g., formalin-fixed paraffin-embedded and circulating free plasma DNA (cfDNA)). Here we describe a comprehensive comparison of bisulfite and enzymatic methylation sequencing, using commercially available assays in clinically relevant patient samples...

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PMID: 41044668
DOI: 10.1186/s13148-025-01959-0

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